To improve yield and robustness of plants, scientists are working with genetic modification. This process tries to artificially mimic the natural evolutionary processes of life, manipulating DNA of an organism by inserting or deleting fragments or by other means, in order to add some particular desirable characteristics.

It is feared that a strain which has been produced artificially may in turn have some other harmful effect on ecology or on humans which will express itself once it is cultivated on a global scale. It is also expected that pathogens which use more natural processes to adapt to the genetically modified crops would produce stronger methods of attack against which the artificially modified forms would have lost their own natural ability to develop competitive strategies.

The Trivedi Effect enhances desired properties of crops in a natural and all-round manner, through a symbiotic transmission of intentional energy which pushes the crop in the preferred direction while also enhancing its own adaptive powers. Experiments in different environments show plant responses which are environment-dependent and which can take care of extreme adverse conditions (for example see section on Mango). The natural ability of the plant to find solutions in a changing environment appears enhanced by this treatment.

Experiments

Four projects on crops, having similar results, are described on this page. The first two were handled by leading agricultural universities in India, while the others were undertaken by reputed research laboratories of private companies.

Agricultural University: Dr. Balasaheb Sawant Konkan Krishi Vidyapeeth (BSKKV), Maharashtra, India (Dapoli)

Six sets of annual vegetable seeds, covering 5 species as above, were divided into two equal portions of which one was treated by the thought intervention of Mr.Trivedi for about 3 minutes, and the second retained as controls. The Treated and Control seeds were sown in adjacent plots so as to have similar soil and aerial environments. Standard cultivation practices were followed for the control plots, with protection against pathogens and pests and additions of supplements with the use of chemical additives, organic fertilizers, pesticides and fungicides along with regular irrigation; whereas treated plots were given only irrigation. Weeding was required in case of the control plants, whereas none was required in treated plots. Experimental details and the report can be seen here.

In the second phase of the project, soil analysis was conducted on control and treated plots with soil samples from four corners and the center of the plots compared. Amazingly, the population of harmful pathogens was found to have decreased and beneficial microbes had increased. There was also change in mineral composition in the treated plot as compared to the control. Further descriptions may be seen here.

Results

To see pictures of individual crops click on the links below:

Over 80% treated seeds germinated in 5-6 days, while control seeds (65% overall) took 10-15 days. Unusually, seeds which had become deeply embedded in the soil germinated by lifting the soil after 18 to 25 days, raising survival rate to 99%. The control plot looked thin and scattered (see pictures) as compared to the treated.

Treated Upper: Groundnuts at day 73	Controls (on same day)Upper: Groundnut
Lower: Cow Pea yield	Lower: Cow Pea yield

Branching was considerably more, as well as yield-related features such as significantly higher siliquae per plant. Colors as well as shapes of leaves and fruit were uniform and glossy and textures firm as compared to the often rougher, sometimes deformed shapes in the controls. Leaves remained dark green and glossy upto the harvesting period with low rates of senescence, i.e. aging, indicating photosynthetic activity till the end.

Chlorophyll assays showed higher “a” & “b” content in the treated crops by 60% & 100% respectively and also a change in Chlorophyl b:a ratio from 1:3 to 1:2. Flowering started nearly 30 days earlier in the treated crop and mature seeds were harvested 8-10 days earlier in the treated plants; the longevity of the treated seedlings increased, extending the fruiting period and adding to an overall higher yield in every case, with ranges between 13% – 500%. The fruit was also usually heavier and larger in size.

The complete data on the Mustard crop is given in Table 1 below. This data is fully consistent with the Mustard data from the BHU project, further described below.

Table 1: Growth, Yield attributes and Chlorophyll content of mustard after seed treatment

Monad Nanotech Pvt. Ltd, India, & DNA testing in India and Canada

With the startling change in growth and immunity clearly visible in most of the plants, it was decided to assay for the major biochemicals responsible for growth and immunity in plants. Auxins are known to control and mediate all stages of growth responses in all plants with a common auxin, IAA (indole-3-acetic acid), having a major role. As a measure of plant immunity, the biochemical Glutathione was selected which correlates well with immunity and especially with stress response in both plants and animals. These tests were conducted by a specialist organization, Monad Nanotech Pvt. Ltd, India.

The changes seen in plant morphology i.e. properties such as the appearance of its leaves or its branching patterns, were suspected to have a genetic basis. To test for changes in genes, the DNA of control and treated species were compared using DNA polymorphism tests. At DNA Landmarks, Canada, 3 species were regrown from control and treated seeds and completely examined using 100 SSR markers, while similar tests on 38 crops were conducted at Bangalore Geneii using 5-8 RAPD markers. The crops analysed included the mustard and other crops selected for growth studies.

To see a discussion of the genetic results, click here. True polymorphism was seen in each case of the 38 crops: 7.8 to 69%.

The full results of 38 crops and 3 crops using 100 SSR markers can also be seen.

Biochemical Parameters

Samples: 5 different species of seeds: Mustard, Cotton, Okra, Soybean, Rice
Date: May 2006

To view test results, click on the links above of individual crops.

The treated and control seeds were soaked in distilled water, germinated in a humid environment and their growth parameters noted. Parameters recorded were consistent with those noted in the field. Concentrations of the glutathione were assayed by harvesting leaves of the young seedlings at the appropriate stage. The rice plants were further allowed to grow to a mature stage in the field and the assays conducted again on leaves.

IAA measures were separately recorded for roots and shoots in the seedlings.

The results consistently showed significantly enhanced levels in both IAA (13% to 107%) as well as Glutathione (28% to 207%), both in the young seedling and in the mature plant (639% in the seedling and 56% in the field). Thus the impact of the energy treatment remained with the seedling when it was transplanted to the field. Incidences of disease were regularly noted in controls but completely absent in treated plants.

Banaras Hindu University, Varanasi, India (BHU)

Photos

In an earlier pilot experiment conducted by the BHU in November 2005, mustard and chick pea seed were treated by the thought transmission of Mr. Trivedi and sown in plots which had also been treated by him. A similar set of untreated controls were planted in the adjacent, untreated plot. The mustard crop was sprayed against the aphid while no protection was given to the chick pea. Similarly no chemical additives were used in any later experiments on plants after this.

Further details of the experiment as also some photos are available at the link above, with a further link to more photographs. There was a startling difference between the lushness of the branching, leaves and canopy of the treated crops versus the controls which were quantified as in the Tables 2 & 3 below.

Table 2: Growth and yield attributes of mustard at BHU farm

*Net plot size 11×4.4=48.4m2

Table 3: Growth, yield and attributes chickpea as influenced by seed and plot treatment.

*Net plot size 11×4.2=46.2m2

To see the original report of the BHU project, click here.

Shahapur, private farm, India

Six species of seeds were treated in August 2006 at a private farm, namely Okra, Bottle Gourd, Sponge Gourd, Bitter Gourd, Maize, Rice. The results can be seen in the photographs of each at the corresponding links.

The Bottle Gourd was the most surprising; despite being a climber the treated plant had strong stems and was erect for the first six weeks, before beginning to climb the trellis. The stems of the Sponge Gourd also showed strength sufficient to be able to hold the fruit up against gravity for a portion of its life.

Diverse responses in changed morphologies were seen across treated crops, with a common finding of greatly improved yield, absolutely no visible incidence of disease or pest attack in any of them and a predominant uniformity of color and shape in the various parts of the treated crops. Textures were often softer or silkier to the touch. In the case of rice, the treated crop was further sprayed with its most common infection, the bacteria, Xanthomonas campestris, and yet showed no instance of succumbing. Control plants however invariably showed incidences of disease, deformities and other weaknesses and rarely came close to the treated plants in perfection of shape and texture.

While on the whole the stems became thicker and stronger, it was observed that the stalk of the okra fruit was tenderer and the fruit could be easily plucked. There was a clear difference in color and vitality of leaves and stems with control plants often showing more yellowish green and an appearance of wilting in the sun compared to the freshness apparent in the treated. The quality of the fruit in all cases was healthy in appearance, enhanced in texture and improved in quality.

Photographs and individual differences can be seen on the web-page of each crop at the above links, while details of the seeds used are available here.